Anand Padmanabhan: Negative ELISA Does Not Rule Out Heparin-Induced Thrombocytopenia
Anand Padmanabhan, Professor and Clinician-Investigator at Mayo Clinic, shared on LinkedIn about a recent article he and his colleagues co-authored, published in American Journal of Hematology, adding:
” New publication in American Journal of Hematology from our group:
Discusses an under-recognized entity, ‘ELISA-negative HIT’ – Patients with pathogenic, platelet-activating anti-PF4 antibodies despite negative HIT ELISA tests
- In a cohort of 500 cases, found missed diagnoses (3/500 ~0.6%) that most standard ELISA assays (with PF4/polyvinyl sulfonate and PF4/Heparin targets) fail to detect
- Clinical impact: Re-exposure to heparin led to platelet drops in all patients ELISA-neg HIT patients and new thrombosis in one
- Takeaway: In infrequent settings, Negative ELISA does not rule-out HIT – functional testing plus clinical judgment remain critical
Congrats to the multi-center team who worked on this study: Adam Kanack, Leben Tefera, Emily Armstrong, Morayma Reyes, Kristi Smock, Ana Casanegra, Curtis Jones, Gretchen Johns, Demin Wang, Renren Wen, Dong Chen, Rajiv Pruthi.”
Title: Pathogenic PF4/Polyanion ELISA-Negative Antibodies in HIT
Authors: Adam J. Kanack, Noah P. Splinter, Emily E. Mauch, Leben Tefera, Morayma Reyes Gil, Sakshi Jasra, Andrew Goodwin, Kristi J. Smock, Hadiyah Ahmad, Aneel Ashrani, Nathaniel L. Robinson, Ana I. Casanegra, Curtis G. Jones, Shannon M. Pechauer, Erin Yttre, Richard H. Aster, Mindy C. Kohlhagen, Rachel R. Leger, Gretchen Johns, David L. Murray, Lu Zhou, Demin Wang, Renren Wen, Dong Chen, Rajiv K. Pruthi, Anand Padmanabhan
Stay updated on all scientific advances with Hemostasis Today.
-
May 12, 2026, 16:46Tagreed Alkaltham: Why Apheresis Matters in Modern Transfusion Medicine
-
May 12, 2026, 16:37Reinhold Kreutz: Cardiovascular Burden in Acute Intermittent Porphyria Needs Greater Awareness
-
May 12, 2026, 16:33Pablo Corral: The Truth About Very Low LDL-Cholesterol
-
May 12, 2026, 16:24Mildred Lundgren: We Must Talk About the Invisible Causes of Stroke
-
May 12, 2026, 16:17Irene Scala: The Sex Disparities In Access to Acute Stroke Treatments In Italy
-
May 12, 2026, 16:04May Nour: UCLA Health Mobile Stroke Unit Becomes The 1st In The World to Perform mCTA In the Field
-
May 12, 2026, 15:57Leonardo Roever: Prognostic Impact of Lipoprotein(a) and CAR in Elderly Acute Ischemic Stroke Patients
-
May 12, 2026, 15:54Bruno Pougault: Prioritizing Laboratory Tests in Resource-Limited Emergency Care
-
May 12, 2026, 15:37Jennifer Holter Chakrabarty: Supporting the Next Generation of Hematology Researchers