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Amir Hesam Karbakhsh: The Science Behind Platelet-Rich Plasma Preparation

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Sep 2, 2026, 14:51

Amir Hesam Karbakhsh: The Science Behind Platelet-Rich Plasma Preparation

Amir Hesam Karbakhsh, Medical Equipment and Sales Specialist at Salamat Tajhiz Barsava, shared a post on LinkedIn:

“The therapeutic layer in PRP isn’t the layer most people picture.

Spin a tube of whole blood and it separates into three distinct bands, not two — density is doing all the work here.

1. Platelet-Poor Plasma (top, approximately 1.025 g/mL)

Mostly water and proteins.

Often discarded or used separately — this is NOT the therapeutic layer, even though it’s the biggest and most visible one.

2. Buffy Coat (thin middle band, approximately 1.06 g/mL)

This is the actual PRP — a concentrated band of platelets and white blood cells.

Many clinical protocols re-spin this layer specifically (a ‘double-spin’ protocol) to concentrate platelets further before injection.

3. Red Blood Cells (bottom, approximately 1.09–1.11 g/mL)

Heaviest layer.

Centrifugal force scales with radius and the square of angular velocity, so denser particles get pushed outward — and settle at the bottom — fastest.

Where it actually goes wrong in practice isn’t the physics, it’s the execution:

Unbalanced tubes vibrate the rotor and can damage the bearings
Over-speeding shears platelets and causes hemolysis, contaminating the very layer you’re trying to concentrate
Drawing off the buffy coat too high or too low dilutes the final platelet concentration

Same lesson as the last few posts: in biomedical devices, the ‘boring’ physical process — not the marketing term on the box — determines whether the output is actually clinically useful.”

Amir Hesam Karbakhsh: The Science Behind Platelet-Rich Plasma Preparation