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Not All TF Activity Assays Measure Tissue Factor – RPTH Journal
Ana Sachetto/LinkedIn
May 8, 2026, 19:15

Not All TF Activity Assays Measure Tissue Factor – RPTH Journal

RPTH Journal shared a post on LinkedIn about a recent article by Ana T.A. Sachetto et al, adding:

“Measuring tissue factor activity in plasma sounds straightforward.

In reality, it’s anything but!

TF-positive extracellular vesicles are increasingly linked to thrombosis, cancer, sepsis, and inflammatory disease, but accurately detecting low circulating TF activity remains a major technical challenge.

A new RPTH methodological study systematically evaluated 3 commercial plasma TF activity assays against the well-established Chapel Hill EV TF activity assay.

What did they find?

All 3 kits detected recombinant TF (Innovin)

  • But sensitivity and specificity varied substantially
  • Plasma background absorbance and hemolysis interfered with measurements
  • Some assays detected large amounts of TF-independent activity
  • Plasma clotting occurred in certain assay systems

The strongest performer?

The Chapel Hill EV TF activity assay remained the most sensitive and specific approach for detecting TF-dependent activity.

Compared with commercial assays, fold increase in LPS-stimulated samples vs controls:

  • Chapel Hill assay: 33-fold
  • Abcam: 11-fold
  • AssaySense: 5-fold

One important message from this paper:

Not all ‘TF activity’ measurements actually reflect tissue factor. Without proper controls, plasma background, EV-independent coagulation activity, and optical interference can all distort interpretation.

A valuable technical contribution for anyone studying extracellular vesicles, coagulation, immunothrombosis, or plasma biomarkers.”

Title: Evaluation of 3 commercial assays for the measurement of tissue factor activity in human plasma

Authors: Ana T.A. Sachetto, Dougald M. Monroe, Nigel Mackman

RPTH Journal

Other articles about RPTH Journal on Hemostasis Today.

Abcam Ana T.A. Sachetto AssaySense Blood cancer Chapel Hill EV TF coagulation Dougald M. Monroe Extracellular Vesicles Health Hematology Hemostasis News Hemostasis Today Immunothrombosis inflammatory disease Innovin Medicine Nigel Mackman plasma biomarkers RPTH Journal Sepsis thrombosis Tissue Factor
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