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Yves Bikoromana: What Every Lab and Clinician Should Know on Pseudothrombocytopenia
Feb 10, 2026, 16:14

Yves Bikoromana: What Every Lab and Clinician Should Know on Pseudothrombocytopenia

Yves Bikoromana, CEO at MedData-Rwanda and Lab Scientist at The University  Teaching Hospital of Kigali-Chuk, shared a post on LinkedIn.

Pseudothrombocytopenia (PTCP): What Every Lab and Clinician Should Know

Pseudothrombocytopenia is a laboratory artifact where platelet counts appear falsely low on automated analyzers, despite the patient having a normal platelet count in vivo. Importantly, it is not a true clinical thrombocytopenia and does not increase bleeding risk.

Why does it happen?

The most common cause is EDTA-dependent platelet clumping. In some individuals, EDTA (the anticoagulant used in routine CBC tubes) exposes platelet surface antigens, leading to antibody-mediated platelet aggregation. Automated analyzers then misinterpret these clumps as leukocytes or exclude them entirely, resulting in a falsely low platelet count.

 Key Laboratory Features

  • Low platelet count on automated CBC
  • Platelet clumps visible on peripheral blood smear
  • Normal platelet count when blood is collected in:
  1.  Sodium citrate
  2. Heparin
  3. Magnesium sulfate (rarely used but effective)

 Clinical Significance

  • Patients are usually asymptomatic
  • No bleeding manifestations
  • Can be mistaken for serious conditions like:
  1.  Immune thrombocytopenic purpura (ITP)
  2.  Bone marrow disorders
  3.  Drug-induced thrombocytopenia

Why recognition matters
Failure to identify PTCP can lead to:

  • Unnecessary platelet transfusions
  • Inappropriate treatments (e.g., steroids, IVIG)
  • Delays in surgeries or invasive procedures
  • Patient anxiety and misdiagnosis

Best Practice Approach

  • Always review the peripheral smear when thrombocytopenia is detected
  • Repeat platelet count using an alternative anticoagulant
  • Correlate laboratory findings with clinical presentation

Take-home message

Pseudothrombocytopenia highlights the importance of laboratory-clinical correlation and the continued value of manual smear examination in modern diagnostics.”

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